MersV1, Main, Exploration, bibRecord, 001867

Chimeric Antisense Oligonucleotide Conjugated to α-Tocopherol

Identifieur interne : 001867 ( Main/Exploration ); précédent : 001866; suivant : 001868

Chimeric Antisense Oligonucleotide Conjugated to α-Tocopherol

Auteurs : Tomoko Nishina [Japon] ; Junna Numata [Japon] ; Kazutaka Nishina [Japon] ; Kie Yoshida-Tanaka [Japon] ; Keiko Nitta [Japon] ; Wenying Piao [Japon] ; Rintaro Iwata [Japon] ; Shingo Ito [Japon] ; Hiroya Kuwahara [Japon] ; Takeshi Wada [Japon] ; Hidehiro Mizusawa [Japon] ; Takanori Yokota [Japon]

Source :

Molecular Therapy. Nucleic Acids [ 2162-2531 ] ; 2015.

RBID : PMC:4345304

Abstract

We developed an efficient system for delivering short interfering RNA (siRNA) to the liver by using α-tocopherol conjugation. The α-tocopherol–conjugated siRNA was effective and safe for RNA interference–mediated gene silencing in vivo. In contrast, when the 13-mer LNA (locked nucleic acid)-DNA gapmer antisense oligonucleotide (ASO) was directly conjugated with α-tocopherol it showed markedly reduced silencing activity in mouse liver. Here, therefore, we tried to extend the 5′-end of the ASO sequence by using 5′-α-tocopherol–conjugated 4- to 7-mers of unlocked nucleic acid (UNA) as a “second wing.” Intravenous injection of mice with this α-tocopherol–conjugated chimeric ASO achieved more potent silencing than ASO alone in the liver, suggesting increased delivery of the ASO to the liver. Within the cells, the UNA wing was cleaved or degraded and α-tocopherol was released from the 13-mer gapmer ASO, resulting in activation of the gapmer. The α-tocopherol–conjugated chimeric ASO showed high efficacy, with hepatic tropism, and was effective and safe for gene silencing in vivo. We have thus identified a new, effective LNA-DNA gapmer structure in which drug delivery system (DDS) molecules are bound to ASO with UNA sequences.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345304

DOI: 10.1038/mtna.2014.72
PubMed: 25584900
PubMed Central: 4345304

Affiliations:

Japon

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Chimeric Antisense Oligonucleotide Conjugated to α-Tocopherol</title>
<author><name sortKey="Nishina, Tomoko" sort="Nishina, Tomoko" uniqKey="Nishina T" first="Tomoko" last="Nishina">Tomoko Nishina</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Numata, Junna" sort="Numata, Junna" uniqKey="Numata J" first="Junna" last="Numata">Junna Numata</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Nishina, Kazutaka" sort="Nishina, Kazutaka" uniqKey="Nishina K" first="Kazutaka" last="Nishina">Kazutaka Nishina</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yoshida Tanaka, Kie" sort="Yoshida Tanaka, Kie" uniqKey="Yoshida Tanaka K" first="Kie" last="Yoshida-Tanaka">Kie Yoshida-Tanaka</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Nitta, Keiko" sort="Nitta, Keiko" uniqKey="Nitta K" first="Keiko" last="Nitta">Keiko Nitta</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Piao, Wenying" sort="Piao, Wenying" uniqKey="Piao W" first="Wenying" last="Piao">Wenying Piao</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Iwata, Rintaro" sort="Iwata, Rintaro" uniqKey="Iwata R" first="Rintaro" last="Iwata">Rintaro Iwata</name>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff3"><institution>Faculty of Pharmaceutical Sciences, Tokyo University of Science</institution>
, Chiba,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ito, Shingo" sort="Ito, Shingo" uniqKey="Ito S" first="Shingo" last="Ito">Shingo Ito</name>
<affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Department of Pharmaceutical Microbiology, Kumamoto University</institution>
, Kumamoto,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kuwahara, Hiroya" sort="Kuwahara, Hiroya" uniqKey="Kuwahara H" first="Hiroya" last="Kuwahara">Hiroya Kuwahara</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Wada, Takeshi" sort="Wada, Takeshi" uniqKey="Wada T" first="Takeshi" last="Wada">Takeshi Wada</name>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff3"><institution>Faculty of Pharmaceutical Sciences, Tokyo University of Science</institution>
, Chiba,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Mizusawa, Hidehiro" sort="Mizusawa, Hidehiro" uniqKey="Mizusawa H" first="Hidehiro" last="Mizusawa">Hidehiro Mizusawa</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff5"><institution>The Center for Brain Integration Research, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yokota, Takanori" sort="Yokota, Takanori" uniqKey="Yokota T" first="Takanori" last="Yokota">Takanori Yokota</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff5"><institution>The Center for Brain Integration Research, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">25584900</idno>
<idno type="pmc">4345304</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345304</idno>
<idno type="RBID">PMC:4345304</idno>
<idno type="doi">10.1038/mtna.2014.72</idno>
<date when="2015">2015</date>
<idno type="wicri:Area/Pmc/Corpus">000003</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000003</idno>
<idno type="wicri:Area/Pmc/Curation">000003</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000003</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000E97</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000E97</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:25584900</idno>
<idno type="wicri:Area/PubMed/Corpus">001725</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001725</idno>
<idno type="wicri:Area/PubMed/Curation">001725</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001725</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001612</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001612</idno>
<idno type="wicri:Area/Ncbi/Merge">001016</idno>
<idno type="wicri:Area/Ncbi/Curation">001016</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001016</idno>
<idno type="wicri:Area/Main/Merge">001872</idno>
<idno type="wicri:Area/Main/Curation">001867</idno>
<idno type="wicri:Area/Main/Exploration">001867</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Chimeric Antisense Oligonucleotide Conjugated to α-Tocopherol</title>
<author><name sortKey="Nishina, Tomoko" sort="Nishina, Tomoko" uniqKey="Nishina T" first="Tomoko" last="Nishina">Tomoko Nishina</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Numata, Junna" sort="Numata, Junna" uniqKey="Numata J" first="Junna" last="Numata">Junna Numata</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Nishina, Kazutaka" sort="Nishina, Kazutaka" uniqKey="Nishina K" first="Kazutaka" last="Nishina">Kazutaka Nishina</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yoshida Tanaka, Kie" sort="Yoshida Tanaka, Kie" uniqKey="Yoshida Tanaka K" first="Kie" last="Yoshida-Tanaka">Kie Yoshida-Tanaka</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Nitta, Keiko" sort="Nitta, Keiko" uniqKey="Nitta K" first="Keiko" last="Nitta">Keiko Nitta</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Piao, Wenying" sort="Piao, Wenying" uniqKey="Piao W" first="Wenying" last="Piao">Wenying Piao</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Iwata, Rintaro" sort="Iwata, Rintaro" uniqKey="Iwata R" first="Rintaro" last="Iwata">Rintaro Iwata</name>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff3"><institution>Faculty of Pharmaceutical Sciences, Tokyo University of Science</institution>
, Chiba,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ito, Shingo" sort="Ito, Shingo" uniqKey="Ito S" first="Shingo" last="Ito">Shingo Ito</name>
<affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Department of Pharmaceutical Microbiology, Kumamoto University</institution>
, Kumamoto,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kuwahara, Hiroya" sort="Kuwahara, Hiroya" uniqKey="Kuwahara H" first="Hiroya" last="Kuwahara">Hiroya Kuwahara</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Wada, Takeshi" sort="Wada, Takeshi" uniqKey="Wada T" first="Takeshi" last="Wada">Takeshi Wada</name>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff3"><institution>Faculty of Pharmaceutical Sciences, Tokyo University of Science</institution>
, Chiba,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Mizusawa, Hidehiro" sort="Mizusawa, Hidehiro" uniqKey="Mizusawa H" first="Hidehiro" last="Mizusawa">Hidehiro Mizusawa</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff5"><institution>The Center for Brain Integration Research, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yokota, Takanori" sort="Yokota, Takanori" uniqKey="Yokota T" first="Takanori" last="Yokota">Takanori Yokota</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff5"><institution>The Center for Brain Integration Research, Tokyo Medical and Dental University</institution>
, Tokyo,<country>Japan</country>
</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Molecular Therapy. Nucleic Acids</title>
<idno type="eISSN">2162-2531</idno>
<imprint><date when="2015">2015</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>We developed an efficient system for delivering short interfering RNA (siRNA) to the liver by using α-tocopherol conjugation. The α-tocopherol–conjugated siRNA was effective and safe for RNA interference–mediated gene silencing <italic>in vivo</italic>
. In contrast, when the 13-mer LNA (locked nucleic acid)-DNA gapmer antisense oligonucleotide (ASO) was directly conjugated with α-tocopherol it showed markedly reduced silencing activity in mouse liver. Here, therefore, we tried to extend the 5′-end of the ASO sequence by using 5′-α-tocopherol–conjugated 4- to 7-mers of unlocked nucleic acid (UNA) as a “second wing.” Intravenous injection of mice with this α-tocopherol–conjugated chimeric ASO achieved more potent silencing than ASO alone in the liver, suggesting increased delivery of the ASO to the liver. Within the cells, the UNA wing was cleaved or degraded and α-tocopherol was released from the 13-mer gapmer ASO, resulting in activation of the gapmer. The α-tocopherol–conjugated chimeric ASO showed high efficacy, with hepatic tropism, and was effective and safe for gene silencing <italic>in vivo</italic>
. We have thus identified a new, effective LNA-DNA gapmer structure in which drug delivery system (DDS) molecules are bound to ASO with UNA sequences.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Kole, R" uniqKey="Kole R">R Kole</name>
</author>
<author><name sortKey="Krainer, Ar" uniqKey="Krainer A">AR Krainer</name>
</author>
<author><name sortKey="Altman, S" uniqKey="Altman S">S Altman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Obika, S" uniqKey="Obika S">S Obika</name>
</author>
<author><name sortKey="Nanbu, D" uniqKey="Nanbu D">D Nanbu</name>
</author>
<author><name sortKey="Hari, Y" uniqKey="Hari Y">Y Hari</name>
</author>
<author><name sortKey="Morio, K" uniqKey="Morio K">K Morio</name>
</author>
<author><name sortKey="In, Y" uniqKey="In Y">Y In</name>
</author>
<author><name sortKey="Ishida, T" uniqKey="Ishida T">T Ishida</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Obika, S" uniqKey="Obika S">S Obika</name>
</author>
<author><name sortKey="Nanbu, D" uniqKey="Nanbu D">D Nanbu</name>
</author>
<author><name sortKey="Hari, Y" uniqKey="Hari Y">Y Hari</name>
</author>
<author><name sortKey="Andoh, J" uniqKey="Andoh J">J Andoh</name>
</author>
<author><name sortKey="Morio, K" uniqKey="Morio K">K Morio</name>
</author>
<author><name sortKey="Doi, T" uniqKey="Doi T">T Doi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Singh, Sk" uniqKey="Singh S">SK Singh</name>
</author>
<author><name sortKey="Koshkin, Aa" uniqKey="Koshkin A">AA Koshkin</name>
</author>
<author><name sortKey="Wengel, J" uniqKey="Wengel J">J Wengel</name>
</author>
<author><name sortKey="Nielsen, P" uniqKey="Nielsen P">P Nielsen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Freier, Sm" uniqKey="Freier S">SM Freier</name>
</author>
<author><name sortKey="Altmann, Kh" uniqKey="Altmann K">KH Altmann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Martin, P" uniqKey="Martin P">P Martin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Seth, Pp" uniqKey="Seth P">PP Seth</name>
</author>
<author><name sortKey="Vasquez, G" uniqKey="Vasquez G">G Vasquez</name>
</author>
<author><name sortKey="Allerson, Ca" uniqKey="Allerson C">CA Allerson</name>
</author>
<author><name sortKey="Berdeja, A" uniqKey="Berdeja A">A Berdeja</name>
</author>
<author><name sortKey="Gaus, H" uniqKey="Gaus H">H Gaus</name>
</author>
<author><name sortKey="Kinberger, Ga" uniqKey="Kinberger G">GA Kinberger</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Murray, S" uniqKey="Murray S">S Murray</name>
</author>
<author><name sortKey="Ittig, D" uniqKey="Ittig D">D Ittig</name>
</author>
<author><name sortKey="Koller, E" uniqKey="Koller E">E Koller</name>
</author>
<author><name sortKey="Berdeja, A" uniqKey="Berdeja A">A Berdeja</name>
</author>
<author><name sortKey="Chappell, A" uniqKey="Chappell A">A Chappell</name>
</author>
<author><name sortKey="Prakash, Tp" uniqKey="Prakash T">TP Prakash</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Monia, Bp" uniqKey="Monia B">BP Monia</name>
</author>
<author><name sortKey="Lesnik, Ea" uniqKey="Lesnik E">EA Lesnik</name>
</author>
<author><name sortKey="Gonzalez, C" uniqKey="Gonzalez C">C Gonzalez</name>
</author>
<author><name sortKey="Lima, Wf" uniqKey="Lima W">WF Lima</name>
</author>
<author><name sortKey="Mcgee, D" uniqKey="Mcgee D">D McGee</name>
</author>
<author><name sortKey="Guinosso, Cj" uniqKey="Guinosso C">CJ Guinosso</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wahlestedt, C" uniqKey="Wahlestedt C">C Wahlestedt</name>
</author>
<author><name sortKey="Salmi, P" uniqKey="Salmi P">P Salmi</name>
</author>
<author><name sortKey="Good, L" uniqKey="Good L">L Good</name>
</author>
<author><name sortKey="Kela, J" uniqKey="Kela J">J Kela</name>
</author>
<author><name sortKey="Johnsson, T" uniqKey="Johnsson T">T Johnsson</name>
</author>
<author><name sortKey="Hokfelt, T" uniqKey="Hokfelt T">T Hökfelt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jiang, K" uniqKey="Jiang K">K Jiang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Crooke, St" uniqKey="Crooke S">ST Crooke</name>
</author>
<author><name sortKey="Geary, Rs" uniqKey="Geary R">RS Geary</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Straarup, Em" uniqKey="Straarup E">EM Straarup</name>
</author>
<author><name sortKey="Fisker, N" uniqKey="Fisker N">N Fisker</name>
</author>
<author><name sortKey="Hedtj Rn, M" uniqKey="Hedtj Rn M">M Hedtjärn</name>
</author>
<author><name sortKey="Lindholm, Mw" uniqKey="Lindholm M">MW Lindholm</name>
</author>
<author><name sortKey="Rosenbohm, C" uniqKey="Rosenbohm C">C Rosenbohm</name>
</author>
<author><name sortKey="Aarup, V" uniqKey="Aarup V">V Aarup</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Koller, E" uniqKey="Koller E">E Koller</name>
</author>
<author><name sortKey="Vincent, Tm" uniqKey="Vincent T">TM Vincent</name>
</author>
<author><name sortKey="Chappell, A" uniqKey="Chappell A">A Chappell</name>
</author>
<author><name sortKey="De, S" uniqKey="De S">S De</name>
</author>
<author><name sortKey="Manoharan, M" uniqKey="Manoharan M">M Manoharan</name>
</author>
<author><name sortKey="Bennett, Cf" uniqKey="Bennett C">CF Bennett</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Juliano, Rl" uniqKey="Juliano R">RL Juliano</name>
</author>
<author><name sortKey="Ming, X" uniqKey="Ming X">X Ming</name>
</author>
<author><name sortKey="Nakagawa, O" uniqKey="Nakagawa O">O Nakagawa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kappus, H" uniqKey="Kappus H">H Kappus</name>
</author>
<author><name sortKey="Diplock, At" uniqKey="Diplock A">AT Diplock</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nishina, K" uniqKey="Nishina K">K Nishina</name>
</author>
<author><name sortKey="Unno, T" uniqKey="Unno T">T Unno</name>
</author>
<author><name sortKey="Uno, Y" uniqKey="Uno Y">Y Uno</name>
</author>
<author><name sortKey="Kubodera, T" uniqKey="Kubodera T">T Kubodera</name>
</author>
<author><name sortKey="Kanouchi, T" uniqKey="Kanouchi T">T Kanouchi</name>
</author>
<author><name sortKey="Mizusawa, H" uniqKey="Mizusawa H">H Mizusawa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Uno, Y" uniqKey="Uno Y">Y Uno</name>
</author>
<author><name sortKey="Piao, W" uniqKey="Piao W">W Piao</name>
</author>
<author><name sortKey="Miyata, K" uniqKey="Miyata K">K Miyata</name>
</author>
<author><name sortKey="Nishina, K" uniqKey="Nishina K">K Nishina</name>
</author>
<author><name sortKey="Mizusawa, H" uniqKey="Mizusawa H">H Mizusawa</name>
</author>
<author><name sortKey="Yokota, T" uniqKey="Yokota T">T Yokota</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hung, G" uniqKey="Hung G">G Hung</name>
</author>
<author><name sortKey="Xiao, X" uniqKey="Xiao X">X Xiao</name>
</author>
<author><name sortKey="Peralta, R" uniqKey="Peralta R">R Peralta</name>
</author>
<author><name sortKey="Bhattacharjee, G" uniqKey="Bhattacharjee G">G Bhattacharjee</name>
</author>
<author><name sortKey="Murray, S" uniqKey="Murray S">S Murray</name>
</author>
<author><name sortKey="Norris, D" uniqKey="Norris D">D Norris</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Soutschek, J" uniqKey="Soutschek J">J Soutschek</name>
</author>
<author><name sortKey="Akinc, A" uniqKey="Akinc A">A Akinc</name>
</author>
<author><name sortKey="Bramlage, B" uniqKey="Bramlage B">B Bramlage</name>
</author>
<author><name sortKey="Charisse, K" uniqKey="Charisse K">K Charisse</name>
</author>
<author><name sortKey="Constien, R" uniqKey="Constien R">R Constien</name>
</author>
<author><name sortKey="Donoghue, M" uniqKey="Donoghue M">M Donoghue</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kuwahara, H" uniqKey="Kuwahara H">H Kuwahara</name>
</author>
<author><name sortKey="Nishina, K" uniqKey="Nishina K">K Nishina</name>
</author>
<author><name sortKey="Yoshida, K" uniqKey="Yoshida K">K Yoshida</name>
</author>
<author><name sortKey="Nishina, T" uniqKey="Nishina T">T Nishina</name>
</author>
<author><name sortKey="Yamamoto, M" uniqKey="Yamamoto M">M Yamamoto</name>
</author>
<author><name sortKey="Saito, Y" uniqKey="Saito Y">Y Saito</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kanasty, R" uniqKey="Kanasty R">R Kanasty</name>
</author>
<author><name sortKey="Dorkin, Jr" uniqKey="Dorkin J">JR Dorkin</name>
</author>
<author><name sortKey="Vegas, A" uniqKey="Vegas A">A Vegas</name>
</author>
<author><name sortKey="Anderson, D" uniqKey="Anderson D">D Anderson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Takeshita, F" uniqKey="Takeshita F">F Takeshita</name>
</author>
<author><name sortKey="Minakuchi, Y" uniqKey="Minakuchi Y">Y Minakuchi</name>
</author>
<author><name sortKey="Nagahara, S" uniqKey="Nagahara S">S Nagahara</name>
</author>
<author><name sortKey="Honma, K" uniqKey="Honma K">K Honma</name>
</author>
<author><name sortKey="Sasaki, H" uniqKey="Sasaki H">H Sasaki</name>
</author>
<author><name sortKey="Hirai, K" uniqKey="Hirai K">K Hirai</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rozema, Db" uniqKey="Rozema D">DB Rozema</name>
</author>
<author><name sortKey="Lewis, Dl" uniqKey="Lewis D">DL Lewis</name>
</author>
<author><name sortKey="Wakefield, Dh" uniqKey="Wakefield D">DH Wakefield</name>
</author>
<author><name sortKey="Wong, Sc" uniqKey="Wong S">SC Wong</name>
</author>
<author><name sortKey="Klein, Jj" uniqKey="Klein J">JJ Klein</name>
</author>
<author><name sortKey="Roesch, Pl" uniqKey="Roesch P">PL Roesch</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kumar, P" uniqKey="Kumar P">P Kumar</name>
</author>
<author><name sortKey="Wu, H" uniqKey="Wu H">H Wu</name>
</author>
<author><name sortKey="Mcbride, Jl" uniqKey="Mcbride J">JL McBride</name>
</author>
<author><name sortKey="Jung, Ke" uniqKey="Jung K">KE Jung</name>
</author>
<author><name sortKey="Kim, Mh" uniqKey="Kim M">MH Kim</name>
</author>
<author><name sortKey="Davidson, Bl" uniqKey="Davidson B">BL Davidson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kumar, P" uniqKey="Kumar P">P Kumar</name>
</author>
<author><name sortKey="Ban, Hs" uniqKey="Ban H">HS Ban</name>
</author>
<author><name sortKey="Kim, Ss" uniqKey="Kim S">SS Kim</name>
</author>
<author><name sortKey="Wu, H" uniqKey="Wu H">H Wu</name>
</author>
<author><name sortKey="Pearson, T" uniqKey="Pearson T">T Pearson</name>
</author>
<author><name sortKey="Greiner, Dl" uniqKey="Greiner D">DL Greiner</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jearawiriyapaisarn, N" uniqKey="Jearawiriyapaisarn N">N Jearawiriyapaisarn</name>
</author>
<author><name sortKey="Moulton, Hm" uniqKey="Moulton H">HM Moulton</name>
</author>
<author><name sortKey="Buckley, B" uniqKey="Buckley B">B Buckley</name>
</author>
<author><name sortKey="Roberts, J" uniqKey="Roberts J">J Roberts</name>
</author>
<author><name sortKey="Sazani, P" uniqKey="Sazani P">P Sazani</name>
</author>
<author><name sortKey="Fucharoen, S" uniqKey="Fucharoen S">S Fucharoen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Prakash, Tp" uniqKey="Prakash T">TP Prakash</name>
</author>
<author><name sortKey="Graham, Mj" uniqKey="Graham M">MJ Graham</name>
</author>
<author><name sortKey="Yu, J" uniqKey="Yu J">J Yu</name>
</author>
<author><name sortKey="Carty, R" uniqKey="Carty R">R Carty</name>
</author>
<author><name sortKey="Low, A" uniqKey="Low A">A Low</name>
</author>
<author><name sortKey="Chappell, A" uniqKey="Chappell A">A Chappell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lennox, Ka" uniqKey="Lennox K">KA Lennox</name>
</author>
<author><name sortKey="Behlke, Ma" uniqKey="Behlke M">MA Behlke</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tabata, K" uniqKey="Tabata K">K Tabata</name>
</author>
<author><name sortKey="Yamaoka, K" uniqKey="Yamaoka K">K Yamaoka</name>
</author>
<author><name sortKey="Kaibara, A" uniqKey="Kaibara A">A Kaibara</name>
</author>
<author><name sortKey="Suzuki, S" uniqKey="Suzuki S">S Suzuki</name>
</author>
<author><name sortKey="Terakawa, M" uniqKey="Terakawa M">M Terakawa</name>
</author>
<author><name sortKey="Hata, T" uniqKey="Hata T">T Hata</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yamaoka, K" uniqKey="Yamaoka K">K Yamaoka</name>
</author>
<author><name sortKey="Tanigawara, Y" uniqKey="Tanigawara Y">Y Tanigawara</name>
</author>
<author><name sortKey="Nakagawa, T" uniqKey="Nakagawa T">T Nakagawa</name>
</author>
<author><name sortKey="Uno, T" uniqKey="Uno T">T Uno</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nishida, Y" uniqKey="Nishida Y">Y Nishida</name>
</author>
<author><name sortKey="Ito, S" uniqKey="Ito S">S Ito</name>
</author>
<author><name sortKey="Ohtsuki, S" uniqKey="Ohtsuki S">S Ohtsuki</name>
</author>
<author><name sortKey="Yamamoto, N" uniqKey="Yamamoto N">N Yamamoto</name>
</author>
<author><name sortKey="Takahashi, T" uniqKey="Takahashi T">T Takahashi</name>
</author>
<author><name sortKey="Iwata, N" uniqKey="Iwata N">N Iwata</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<affiliations><list><country><li>Japon</li>
</country>
</list>
<tree><country name="Japon"><noRegion><name sortKey="Nishina, Tomoko" sort="Nishina, Tomoko" uniqKey="Nishina T" first="Tomoko" last="Nishina">Tomoko Nishina</name>
</noRegion>
<name sortKey="Ito, Shingo" sort="Ito, Shingo" uniqKey="Ito S" first="Shingo" last="Ito">Shingo Ito</name>
<name sortKey="Iwata, Rintaro" sort="Iwata, Rintaro" uniqKey="Iwata R" first="Rintaro" last="Iwata">Rintaro Iwata</name>
<name sortKey="Iwata, Rintaro" sort="Iwata, Rintaro" uniqKey="Iwata R" first="Rintaro" last="Iwata">Rintaro Iwata</name>
<name sortKey="Kuwahara, Hiroya" sort="Kuwahara, Hiroya" uniqKey="Kuwahara H" first="Hiroya" last="Kuwahara">Hiroya Kuwahara</name>
<name sortKey="Kuwahara, Hiroya" sort="Kuwahara, Hiroya" uniqKey="Kuwahara H" first="Hiroya" last="Kuwahara">Hiroya Kuwahara</name>
<name sortKey="Mizusawa, Hidehiro" sort="Mizusawa, Hidehiro" uniqKey="Mizusawa H" first="Hidehiro" last="Mizusawa">Hidehiro Mizusawa</name>
<name sortKey="Mizusawa, Hidehiro" sort="Mizusawa, Hidehiro" uniqKey="Mizusawa H" first="Hidehiro" last="Mizusawa">Hidehiro Mizusawa</name>
<name sortKey="Nishina, Kazutaka" sort="Nishina, Kazutaka" uniqKey="Nishina K" first="Kazutaka" last="Nishina">Kazutaka Nishina</name>
<name sortKey="Nishina, Kazutaka" sort="Nishina, Kazutaka" uniqKey="Nishina K" first="Kazutaka" last="Nishina">Kazutaka Nishina</name>
<name sortKey="Nishina, Tomoko" sort="Nishina, Tomoko" uniqKey="Nishina T" first="Tomoko" last="Nishina">Tomoko Nishina</name>
<name sortKey="Nitta, Keiko" sort="Nitta, Keiko" uniqKey="Nitta K" first="Keiko" last="Nitta">Keiko Nitta</name>
<name sortKey="Numata, Junna" sort="Numata, Junna" uniqKey="Numata J" first="Junna" last="Numata">Junna Numata</name>
<name sortKey="Piao, Wenying" sort="Piao, Wenying" uniqKey="Piao W" first="Wenying" last="Piao">Wenying Piao</name>
<name sortKey="Piao, Wenying" sort="Piao, Wenying" uniqKey="Piao W" first="Wenying" last="Piao">Wenying Piao</name>
<name sortKey="Wada, Takeshi" sort="Wada, Takeshi" uniqKey="Wada T" first="Takeshi" last="Wada">Takeshi Wada</name>
<name sortKey="Wada, Takeshi" sort="Wada, Takeshi" uniqKey="Wada T" first="Takeshi" last="Wada">Takeshi Wada</name>
<name sortKey="Yokota, Takanori" sort="Yokota, Takanori" uniqKey="Yokota T" first="Takanori" last="Yokota">Takanori Yokota</name>
<name sortKey="Yokota, Takanori" sort="Yokota, Takanori" uniqKey="Yokota T" first="Takanori" last="Yokota">Takanori Yokota</name>
<name sortKey="Yokota, Takanori" sort="Yokota, Takanori" uniqKey="Yokota T" first="Takanori" last="Yokota">Takanori Yokota</name>
<name sortKey="Yoshida Tanaka, Kie" sort="Yoshida Tanaka, Kie" uniqKey="Yoshida Tanaka K" first="Kie" last="Yoshida-Tanaka">Kie Yoshida-Tanaka</name>
<name sortKey="Yoshida Tanaka, Kie" sort="Yoshida Tanaka, Kie" uniqKey="Yoshida Tanaka K" first="Kie" last="Yoshida-Tanaka">Kie Yoshida-Tanaka</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001867 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001867 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:4345304
   |texte=   Chimeric Antisense Oligonucleotide Conjugated to α-Tocopherol
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:25584900" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a MersV1

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

Serveur d'exploration MERS

Chimeric Antisense Oligonucleotide Conjugated to α-Tocopherol

Chimeric Antisense Oligonucleotide Conjugated to α-Tocopherol

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.